Sterilization 2017-04-24T14:04:26+00:00

What is Sterilization?

Any process that effectively kills or eliminates transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.) from a surface, equipment.

 How much temperature and time is required to sterilize instruments?

To achieve sterility, a holding time of at least 15 minutes at 121 °C or 3 minutes at 134 °C is required.  This treatment will inactivate all fungi, bacteria, viruses and also bacterial spores, which can be quite resistant. (Source:

 How is Sterilization achieved at Apex?

Sterilization at Apex is achieved using autoclave shown below.



Autoclave cycle parameters at Apex:

Pressure (kgf/cm2) Material Time of Sterilization Time of Drying
2.0 +/- 0.1 Metal Instruments 4 Minutes 12 Minutes 134 °C
2.0 +/- 0.1 Wrapped Metal Instruments 5 Minutes 12 Minutes 134 °C
2.0 +/- 0.1 Textile Articles 12-16 Minutes 12 Minutes 134 °C
1.2 +/- 0.1 Metal Instruments 14-16 Minutes 12 Minutes 134 °C
1.2 +/- 0.1 Textile Articles 12-16 Minutes 12 Minutes 134 °C


What are the steps involved in sterilization process at Apex Dental Centre?


Step-1 : First we clean all the instruments thoroughly with hot water and then with spirit.


Step-2 : Hand-pieces and similar instruments are cleaned externally with spirit and internally with spray.


Step-3 : All instruments are kept in a ultrasonic cleaner to remove blood & fluid stains from inaccessible grooves. This is achieved using high frequency ultrasonic vibrations



Step-4 : All the cleaned equipment are sealed in a pouch.  Each pouch contains set of instruments required for single patient.


 Step-5 :  All pouches sealed in step-3 are kept in autoclave. Autoclave is run for one cycle with parameters mentioned above. This ensures every corner of the instrument is sterilized.


Step-6 : After Autoclave cycle,  sealed pouches are kept in a UV chamber till they are taken out for use.  Ultraviolet rays in the chamber ensure that instruments remain in sterile condition when they are taken out for use on you.